- Reactive to Aflatoxin B₁, B₂, G₁, G₂ and Sterigmatocystin
- Enable to clean-up Aflatoxins and Sterigmatocystin in small amount of extracts, it helps shortening the time of pre-treatments
- The tolerance on organic solvents has been improved Applicable organic solvent concentration rate;
Clean-up of Aflatoxins:
20% concentration of Acetonitrile
40% concentration of Methanol
Clean-up of Sterigmatocystin:
10% concentration of Acetonitrile
20% concentration of Methanol
What is Immunoaffinity Column; IAC?
An IAC is a column with an antibody immobilized in gels designed for clean-ups. An IAC offers outstanding selectivity because it uses the property of the antibody to bond to a specific antigen. It is also able to efficiently recover the target substance, resulting in excellent clean-up capability.
The monoclonal antibody immobilized in gels is filled in the column. Make bound Aflatoxins / Sterigmatocystin with the antibody by adding the extract of organic solvents, such as foodstuffs, to the column after diluting the extract to predetermined concentration. And, clean-up the left substances by running purified water. Make elute of Aflatoxins and Sterigmatocystin with dissociation of these from the antibody by adding the organic solvent such as Acetonitrile. It enables to concentrate and to clean-up high purified Aflatoxins and Sterigmatocystin in the extract of foodstuffs by these operations.